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Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) <t>ASCA</t> titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.
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Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) <t>ASCA</t> titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.
Serological Determination For Anti Saccharomycescerevisiae (Asca) Iga And Igg, supplied by Prometheus Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) <t>ASCA</t> titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.
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Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) <t>ASCA</t> titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.
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asca assays asca igg  (ORGENTEC Diagnostika GmbH)
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Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) <t>ASCA</t> titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.
Asca Assays Asca Igg, supplied by ORGENTEC Diagnostika GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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asca igg elisa kit  (Abnova)
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Abnova asca igg elisa kit
Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) <t>ASCA</t> titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.
Asca Igg Elisa Kit, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) ASCA titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.

Journal: Pathogens and Disease

Article Title: Typing of feces-derived Candida albicans strains using a novel seven-locus microsatellite panel reveals associations with yeast phenotype in individuals with inflammatory bowel disease

doi: 10.1093/femspd/ftaf001

Figure Lengend Snippet: Enzymatic activity of feces-derived C. albicans strains reveals no differentially active enzymes. (A–E) Enzyme activity was based in triplicate for one strain per individual. Each data point represents mean activity of a strain as determined in triplicate. (A) Proteinase activity. (B) Phospholipase activity. (C) Lipase activity. (D) Esterase activity. (E) Average activity across all enzymes. Horizontal dashed line indicates activity of reference strain SC5314. (F–J) Clinical fecal and serum titers. (F) Fecal calprotectin. (G) ASCA titers, IgA immunoglobulins. (H) ASCA titers, IgG immunoglobulins. (I) Total ASCA titers. (J) Serum 1,3-β-glucan levels. (K) Correlation plot of all determined enzyme activities and clinical parameters based on Spearman r . Letters and symbols denote significance levels. Spearman r , a P < .05, b P < .005. (L) Correlation between PC2 and proteinase activity. Spearman r = 0.417, P = .043, n = 24. (M) Correlation between esterase activity and PC2. Spearman r = .408, P = .048, n = 24. Data of all included patients, except for enzyme activity ( n = 8 HV, n = 12 CD, n = 4 UC). Kruskal–Wallis or Mann–Whitney U test, * P < .05, ** P < .01.

Article Snippet: For determination of ASCAs and 1,3-β-glucans in serum, colorimetric kits were used according to the manufacturer's protocol, being the Orgentec ASCA IgA/IgG kit (Siemens Healthineers, Den Haag, The Netherlands) and FungiTell diagnostic kit (Nodia, Amstelveen, The Netherlands), respectively.

Techniques: Activity Assay, Derivative Assay, MANN-WHITNEY